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Table 1 DNA reads obtained by Roche 454 sequencing of different samples

From: Metabarcoding of the kombucha microbial community grown in different microenvironments

Sample Total number of reads before and after filtering and chimera removal Total length, bp Average Min. read lengtha Max. read length Sobs/S exp
sBTS
 Pellicle: 16S 2,384/2,356 1,123,074 471 77 607 14/46
 Pellicle: ITS 532/530 277,232 521 61 568 5/10
BTH
 Pellicle: 16S 2,632/2,626 1,244,214 472 47 828 14/46
 Pellicle: ITS 7,888/7,783 3,303,150 418 43 561 23/87
nsBTS
 Pellicle: 16S 1,880/1,828 870,798 463 65 563 24/33
 Pellicle: ITS 3,741/2,310 1,138,925 304 41 536 7/23
Hybrid KMC
 Pellicle: 16S 8,716/8,250 2,975,027 341 40 513 9/10
 Pellicle: ITS 7,943/7,113 2,500,278 314 40 541 18/34
 Liquid phase: 16S 6,494/6,325 2,294,949 353 40 513 16/17
 Liquid phase: ITS 9,541/8,281 3,165,774 331 40 521 26/38
  1. aAll reads shorter than 100 bp were filtered out.
  2. S obs observed number of species including those identified by a single read, S exp expected number of species according to Chao estimation (Eq. 1).