Metabarcoding of the kombucha microbial community grown in different microenvironments

AMB Express

Table 1 DNA reads obtained by Roche 454 sequencing of different samples

Sample	Total number of reads before and after filtering and chimera removal	Total length, bp	Average	Min. read length^a	Max. read length	S_obs/S ^†_exp
sBTS
Pellicle: 16S	2,384/2,356	1,123,074	471	77	607	14/46
Pellicle: ITS	532/530	277,232	521	61	568	5/10
BTH
Pellicle: 16S	2,632/2,626	1,244,214	472	47	828	14/46
Pellicle: ITS	7,888/7,783	3,303,150	418	43	561	23/87
nsBTS
Pellicle: 16S	1,880/1,828	870,798	463	65	563	24/33
Pellicle: ITS	3,741/2,310	1,138,925	304	41	536	7/23
Hybrid KMC
Pellicle: 16S	8,716/8,250	2,975,027	341	40	513	9/10
Pellicle: ITS	7,943/7,113	2,500,278	314	40	541	18/34
Liquid phase: 16S	6,494/6,325	2,294,949	353	40	513	16/17
Liquid phase: ITS	9,541/8,281	3,165,774	331	40	521	26/38

^aAll reads shorter than 100 bp were filtered out.
^† S _obs observed number of species including those identified by a single read, S _exp expected number of species according to Chao estimation (Eq. 1).