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AMB Express

Table 2 Kinetic parameters of biomass degrading enzymes

From: Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras

Constructs

Substrate, β-Glucan

Substrate, 4-NP-β-d-GP

KM (%)

vmax (U/mg)

KM (mM)

vmax (U/mg)

Cel5A

0.12

1,816.9

–

–

Bgl1

–

–

0.41

2,031.2

CB

0.23

822.6

0.53

744.0 (1,256.7)a

BC

0.13

1,205.9

0.53

1,472.3 (2,470.3)a

CBE395G

0.25

77.2 (191.1)b

–

–

BE395GC

0.18

216.6 (536.1)b

–

–

  1. aCatalytic activity of Bgl1 calculated under consideration of single enzyme’s molecular weight in fusion construct.
  2. bCatalytic activity of Cel5A calculated under consideration of single enzyme’s molecular weight in fusion construct.
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