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Figure 7 | AMB Express

Figure 7

From: Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras

Figure 7

HPLC-analyses to investigate β-glucan degradation. a Schematic diagram depicting endoglucanase (Cel5A)-mediated degradation of β-glucan (bG) to give oligosaccharides (Os) and cellobiose (Cb). The β-glucosidase Bgl1 converts cellobiose to glucose (Glu). b Quantitative detection of released sugar components from 0.5% (w/v): β-glucan degradation by Cel5A, mixture of Cel5A and Bgl1 (Mix) and fusion constructs. Enzymes were used in 1:1 ratio in all assays. Concentration of fusion constructs was determined based on molecular weight of Cel5A moiety. c Quantitative detection of released glucose from 0.5% (w/v) cellobiose degradation by Bgl1, mixture of Cel5A and Bgl1 (Mix) and fusion constructs. Enzymes were used in 1:1 ratio in all assays. Concentration of fusion constructs was determined based on molecular weight of Bgl1 moiety. Colour code of sugar components in a, b and c: bG black, Os light grey, Cb grey, Glu dark grey.

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