Inoculation and fermentation of
in 2.5 L benchtop bioreactor. A. Direct inoculation of the benchtop fermenter using a serum bottle with preculture. Nitrogen was introduced into the serum bottle via a small filter creating excess pressure inside the bottle. A second tube was used to channel the preculture directly into the inoculum device. B. Foam-free cultivation of Bacillus subtilis DSM 10T applying an anaerobic fermentation process.