Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass

AMB Express

Table 1 Identification and characterization of bacterial isolates based on 16S rDNA homology analysis and cellulolytic activity

Strain	GenBank/DSMZ accession no.*	Bacteria	Closest relative	16S rDNA identity (%)	Cellulolytic activity
Strain	GenBank/DSMZ accession no.*	Bacteria	Closest relative	16S rDNA identity (%)	Bagasse	HT	CMC (EIA)
Kd70 TUC-EEAOC	KM096608/DSM 27019	Klebsiella oxytoca	K. oxytoca KCTC1686	99.0	+	+	+ (5.0)
KdB5 TUC-EEAOC	KM096599	Klebsiella. pneumoniae	K. pneumoniae MGH78578	99.0	+	+	+ (5.5)
KdB1 TUC-EEAOC	KM096598/DSM 27017	Klebsiella variicola	K. variicola At-22	99.7	+	+	+ (5.0)
Kd3 TUC-EEAOC	KM096600	Stenotrophomonas maltophilia	S. maltophilia R551-3	98.3	-	-	+ (2.5)
Kd46 TUC-EEAOC	KM096602	Stenotrophomonas rhizophila	S. rhizophila ep-10	99.0	-	-	+ (2.5)
Kd109 TUC-EEAOC	KM096605/DSM 27021	Bacillus pumilus	B. pumilus SAFR032	99.5	+	+	+ (7.0)
Kd7 TUC-EEAOC	KM096606/DSM 27018	Enterococcus casseliflavus	E. casseliflavus EC-20	99.0	+ weak	+ weak	+ (2.5)
KdL49 TUC-EEAOC	KM096603	Microbacterium hominis	M. hominis DSM12509	99.8	-	-	+ (1.5) weak
KdL45 TUC-EEAOC	KM096601	Microbacterium schleiferi	M. schleiferi DSM20489	97.0	-	-	+ (1.5) weak

Numbers in parenthesis in the column of cellulolytic activity on CMC indicate diameter of halo in centimeters.