Chemical
|
Concentration
|
Biomass state
|
Time
|
Initial PHB content
|
Purity
|
Recovery
|
Mass balance
|
HB/PHB
c
|
---|
[−]
|
[M;w/v%]
|
[−]
|
[h]
|
[wt%]
|
[%]
|
[%]
|
[%]
|
[%]
|
NaOH
|
0.02
|
Fresh
|
1
|
71.8 ± 5.7
|
77.3 ± 4.0
|
92.2 ± 4.2
|
−1.0 ± 3.7
|
97.9 ± 5.3
|
NaOH
|
0.05
|
Fresh
|
1
|
72.7 ± 7.1
|
84.0 ± 1.4
|
94.7 ± 3.2
|
−3.9 ± 2.9
|
96.7 ± 4.2
|
NaOH
|
0.10
|
Fresh
|
1
|
65.3 ± 3.1
|
83.8 ± 4.4
|
98.0 ± 1.4
|
−1.0 ± 1.4
|
92.1 ± 8.5
|
NaOH
|
0.20
|
Fresh
|
1
|
69.4 ± 1.1
|
86.6 ± 3.0
|
96.7 ± 1.9
|
−0.7 ± 2.6
|
97.5 ± 16.7
|
NaOH
|
0.20
|
Fresh
|
0.3
|
68.6 ± 0.7
|
87.3 ± 2.2
|
96.4 ± 2.6
|
−2.9 ± 2.4
|
85.9 ± 14.8
|
NaOH
|
0.20
|
Fresh
|
0.5
|
68.6 ± 0.7
|
88.8 ± 0.8
|
98.5 ± 1.8
|
−0.7 ± 1.8
|
90.9 ± 18.9
|
NaOH
|
0.20
|
Fresh
|
3
|
68.6 ± 0.7
|
92.1 ± 0.8
|
93.5 ± 2.4
|
−1.5 ± 0.6
|
92.0 ± 3.2
|
NaOH
|
0.40
|
Fresh
|
1
|
65.3 ± 3.1
|
87.9 ± 5.4
|
95.2 ± 3.7
|
0.7 ± 3.1
|
89.9 ± 4.8
|
NaOH
|
0.70
|
Fresh
|
1
|
65.3 ± 3.1
|
89.7 ± 5.8
|
90.9 ± 5.0
|
0.4 ± 4.4
|
89.4 ± 7.1
|
NaOH
|
1.00
|
Fresh
|
1
|
65.3 ± 3.1
|
90.6 ± 4.7
|
85.6 ± 2.3
|
−0.3 ± 2.1
|
89.1 ± 4.0
|
NH4OH
|
0.20
|
Fresh
|
1
|
68.6 ± 0.7
|
62.6 ± 2.8
|
63.3 ± 16.4
|
−3.9 ± 0.9
|
36.3 ± 10.9
|
SDS
|
0.20
|
Fresh
|
1
|
68.0 ± 0.0
|
79.0 ± 1.4
|
63.5 ± 0.7
|
3.6 ± 0.6
|
14.0 ± 1.4
|
NaOH + SDS
|
0.20 + 0.025
|
Fresh
|
1
|
66.1 ± 2.2
|
94.9 ± 2.6
|
92.6 ± 6.9
|
−2.7 ± 3.8
|
94.2 ± 6.4
|
NaOH + SDS
|
0.20 + 0.050
|
Fresh
|
1
|
66.1 ± 2.2
|
96.9 ± 1.3
|
93.5 ± 4.8
|
−2.4 ± 2.4
|
92.4 ± 3.1
|
NaOH + SDS
|
0.20 + 0.100
|
Fresh
|
1
|
66.1 ± 2.2
|
98.3 ± 0.5
|
91.5 ± 5.9
|
−3.9 ± 4.8
|
96.3 ± 4.8
|
NaOH + SDS
|
0.20 + 0.200
|
Fresh
|
1
|
66.1 ± 2.2
|
99.1 ± 0.5
|
91.0 ± 4.9
|
−3.1 ± 1.9
|
92.5 ± 5.0
|
NaOH
|
0.20
|
Freezing
|
1
|
65.9 ± 2.4
|
94.1 ± 3.5
|
95.6 ± 2.5
|
−2.9 ± 2.1
|
94.3 ± 5.4
|
NaOH
|
0.20
|
Freeze dried
|
1
|
69.9 ± 2.2
|
95.9 ± 3.7
|
95.5 ± 0.6
|
−3.2 ± 0.8
|
98.8 ± 0.9
|
NH4OH
|
0.20
|
Freeze dried
|
1
|
69.9 ± 2.2
|
87.4 ± 2.1
|
95.0 ± 1.8
|
−3.9 ± 0.9
|
87.1 ± 12.9
|
SDS
|
0.20
|
Freeze dried
|
1
|
69.9 ± 2.2
|
93.5 ± 4.1
|
93.7 ± 2.2
|
−3.1 ± 1.6
|
91.3 ± 8.7
|
CH2Cl2
|
30a
|
Freeze dried
|
o/nb
|
72.2 ± 0.4
|
97.6
|
55.9
|
ND
|
ND
|
-
a30 times of TSS.
-
bOvernight.
-
cFraction of hydrolyzed monomer in total polymer in the supernatant.