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AMB Express

Table 1 Summary of all experiments conducted in this study

From: Feasibility study of an alkaline-based chemical treatment for the purification of polyhydroxybutyrate produced by a mixed enriched culture

Chemical Concentration Biomass state Time Initial PHB content Purity Recovery Mass balance HB/PHB c
[−] [M;w/v%] [−] [h] [wt%] [%] [%] [%] [%]
NaOH 0.02 Fresh 1 71.8 ± 5.7 77.3 ± 4.0 92.2 ± 4.2 −1.0 ± 3.7 97.9 ± 5.3
NaOH 0.05 Fresh 1 72.7 ± 7.1 84.0 ± 1.4 94.7 ± 3.2 −3.9 ± 2.9 96.7 ± 4.2
NaOH 0.10 Fresh 1 65.3 ± 3.1 83.8 ± 4.4 98.0 ± 1.4 −1.0 ± 1.4 92.1 ± 8.5
NaOH 0.20 Fresh 1 69.4 ± 1.1 86.6 ± 3.0 96.7 ± 1.9 −0.7 ± 2.6 97.5 ± 16.7
NaOH 0.20 Fresh 0.3 68.6 ± 0.7 87.3 ± 2.2 96.4 ± 2.6 −2.9 ± 2.4 85.9 ± 14.8
NaOH 0.20 Fresh 0.5 68.6 ± 0.7 88.8 ± 0.8 98.5 ± 1.8 −0.7 ± 1.8 90.9 ± 18.9
NaOH 0.20 Fresh 3 68.6 ± 0.7 92.1 ± 0.8 93.5 ± 2.4 −1.5 ± 0.6 92.0 ± 3.2
NaOH 0.40 Fresh 1 65.3 ± 3.1 87.9 ± 5.4 95.2 ± 3.7 0.7 ± 3.1 89.9 ± 4.8
NaOH 0.70 Fresh 1 65.3 ± 3.1 89.7 ± 5.8 90.9 ± 5.0 0.4 ± 4.4 89.4 ± 7.1
NaOH 1.00 Fresh 1 65.3 ± 3.1 90.6 ± 4.7 85.6 ± 2.3 −0.3 ± 2.1 89.1 ± 4.0
NH4OH 0.20 Fresh 1 68.6 ± 0.7 62.6 ± 2.8 63.3 ± 16.4 −3.9 ± 0.9 36.3 ± 10.9
SDS 0.20 Fresh 1 68.0 ± 0.0 79.0 ± 1.4 63.5 ± 0.7 3.6 ± 0.6 14.0 ± 1.4
NaOH + SDS 0.20 + 0.025 Fresh 1 66.1 ± 2.2 94.9 ± 2.6 92.6 ± 6.9 −2.7 ± 3.8 94.2 ± 6.4
NaOH + SDS 0.20 + 0.050 Fresh 1 66.1 ± 2.2 96.9 ± 1.3 93.5 ± 4.8 −2.4 ± 2.4 92.4 ± 3.1
NaOH + SDS 0.20 + 0.100 Fresh 1 66.1 ± 2.2 98.3 ± 0.5 91.5 ± 5.9 −3.9 ± 4.8 96.3 ± 4.8
NaOH + SDS 0.20 + 0.200 Fresh 1 66.1 ± 2.2 99.1 ± 0.5 91.0 ± 4.9 −3.1 ± 1.9 92.5 ± 5.0
NaOH 0.20 Freezing 1 65.9 ± 2.4 94.1 ± 3.5 95.6 ± 2.5 −2.9 ± 2.1 94.3 ± 5.4
NaOH 0.20 Freeze dried 1 69.9 ± 2.2 95.9 ± 3.7 95.5 ± 0.6 −3.2 ± 0.8 98.8 ± 0.9
NH4OH 0.20 Freeze dried 1 69.9 ± 2.2 87.4 ± 2.1 95.0 ± 1.8 −3.9 ± 0.9 87.1 ± 12.9
SDS 0.20 Freeze dried 1 69.9 ± 2.2 93.5 ± 4.1 93.7 ± 2.2 −3.1 ± 1.6 91.3 ± 8.7
CH2Cl2 30a Freeze dried o/nb 72.2 ± 0.4 97.6 55.9 ND ND
  1. a30 times of TSS.
  2. bOvernight.
  3. cFraction of hydrolyzed monomer in total polymer in the supernatant.
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