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Figure 2 | AMB Express

Figure 2

From: Fe(III) mineral reduction followed by partial dissolution and reactive oxygen species generation during 2,4,6-trinitrotoluene transformation by the aerobic yeast Yarrowia lipolytica

Figure 2

ESR spectra of DEPMPO spin adducts generated in the presence of yeast cells of Y. lipolytica AN-L15 and TNT in the absence of Fe(III) (oxyhydr)oxides (measured 30 minutes after the start of the experiment) (A) and in a standard xanthine/xanthine oxidase system (reference spectrum for DEPMPO-OOH). (B) Simulated spectrum of DEPMPO-OOH is presented as a reference (dashed line); the typical set of hyperfine coupling constants was used for simulation (Frejaville et al. 1995). The spectral lines attributed to DEPMPO-OH spin adduct are marked with asterisks; their appearance is the result of DEPMPO-OOH decomposition to DEPMPO-OH (Stenuit et al. 2012). (C) Same as in (A) with the exception that superoxide dismutase (200 U mL−1) was added. Control experiments in the presence of Y. lipolytica AN-L15 and absence of TNT (D) and in the absence of Y. lipolytica AN-L15 and presence of TNT (E). The spectra were recorded for 10 min after the DEPMPO was added. The experimental parameters were as follows: microwave power 2 mW, microwave frequency 9.73 GHz, modulation amplitude 0.2 mT, time constant 82 ms and scan rate 2.1 G/s.

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