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AMB Express

Table 2 Oligonucleotide primers used in this study

From: Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing

Primer name

Primer sequence (5′-3′)

Restriction sites*

kanMX_FPS1_F

TATTTTCGATCAGATCTCATAGTGAGAAGGCGCAATT CAGTAGTTGGCATCAGAGCAGATTGTACTGAGAG

 

kanMX_R

AAACTCACCGAGGCAGTTCCATAG

 

kanMX_F

ATGGTCAGACTAAACTGGCTGACG

 

kanMX_FPS1_R

CATCCATGCGATACATCATGTATAGTAGGTGACCAGGCTGAGTTC TACCGCCTTTGAGTGAGCTGATAC

 

UTR1_F

CGGGATCC CGAAAACAATGAAGGAGAATGACATGAATA

Bam HI

UTR1_R

CGGAATTC CGGTAACATTATACTGAAAACCTTGCTTGA

Eco RI

GapN_F

CGGGATCC CGAAAACAATGACAAAACAATACAAAAACT

Bam HI

GapN_R

CCATCGAT GGAGATCTTCACTTTATGTCAAAGACAACA

Cla I

  1. Primer overhangs containing part of FPS1 up/down sequences are depicted in bold letters.
  2. *Restriction sites with corresponding restriction enzyme are represented in italics.
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