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AMB Express

Table 2 Growth on different carbon sources

From: L-citrulline production by metabolically engineered Corynebacterium glutamicum from glucose and alternative carbon sources

C. glutamicumstrain

Carbon source concentration

Maximum OD600

Growth rate (h−1)

CIT1(pEKEx3-xylAB)

Xylose: 15 g/L

6 ± 1

0.03 ± 0.01

CIT1(pEKEx3-nagB)

Glucosamine: 10 g/L

3 ± 1

0.02 ± 0.01

CIT1(pAMY)

Soluble starch: 10 g/L Glucose: 2.5 g/L

9 ± 1

0.21 ± 0.01

CIT1(pEC-XT99A)

Soluble starch: 10 g/L Glucose: 2.5 g/L

3 ± 1

0.10 ± 0.01

  1. Fermentations were performed in CGXII minimal medium containing the respective carbon source and were supplemented by 750 μM L-arginine. 1 mM IPTG and 25 μg/ml kanamycin and spectinomycin were added. Values and error bars represent the mean and the standard error of triplicates.
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