(S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB’) from fatty acid degradation operon of Ralstonia eutropha H16

AMB Express

Table 2 Enzyme characteristic of FadB’ in comparison with other enzymes

	Cofactor		Trifunctional activity	Specificity to (S)- or (R)-3-hydroxyacyl-CoA	Reference
	NAD⁺	NADP⁺	Trifunctional activity	Specificity to (S)- or (R)-3-hydroxyacyl-CoA	Reference
FadB’_Re	100^*	5^*	+	S^**	this work
PhaB1_Re	20	100	-	R	Haywood et al. [1988b]
Unidentified acetoacetyl-CoA reductase (R. eutropha H16)	100	2	n.a.	S/R	Haywood et al. [1988b]
FadB_Ec	+	n.a.	+	S	Binstock and Schulz [1981]

*-The activity of FadB’ was measured at 340 nm and 30°C with acetoacetyl-CoA in direction of 3-hydroxybutyryl-CoA formation as described in “Materials and Methods”.
**-The stereospecificity of FadB’ was measured at 303 nm and 30°C with (S)- and (R)-3hydroxybutyryl-CoA as described in “Materials and Methods”.