Table 1 List of primers used in this study
Primer | Nucleotide sequence (5’-3’) | Target ORF |
|---|---|---|
Real-time PCR experiments | ||
YER034W-F | CACCCAGTGATAGAAAGTACCG | YER034W |
YER034W-R | TCCTCATAGTACCTGTCCTCG | YER034W |
prm5-F | GCAATTTTTGCGGGTTTCCTTA | PRM5 (YIL117C) |
prm5-R | TCGTCGCTCGATAACGGTA | PRM5 (YIL117C) |
YBR269C-F | TCGCAAGAGGCAATAGATCAG | FMP21 (YBR269C) |
YBR269C-R | TCGAATTCAGGTATGGTCTTGG | FMP21 (YBR269C) |
Act1-F | AGCCTTCTACGTTTCCATCC | ACT1 |
Act1-R | CTTTCAGCAGTGGTGGAGAA | ACT1 |
Construction of the FMP21-overexpressing strain | ||
URA3-F | CAGGGTCCATAAAGCTTT | |
URA3-R | TTTATAAAGGCCATGAAGCT | |
TDH3 pro-F | GGAAAGAAAAAGCTTCATGGCCTTTATAAAAACACGCTTTTTCAGTTC | |
TDH3 pro-R | TTTGTTTGTTTATGTGTGTT | |
FMP21-A | AACGCACTCAAGGTTTTTGG | |
FMP21-B | AAGATGAATTGAAAAGCTTTATGGACCCTGTAGTTCTTCTTTTTATTATT | |
FMP21-C | GTTTCGAATAAACACACATAAACAAACAAAATGTTGTGCGCCATCAAAAG | |
FMP21-D | GTATGAATGTGCCCAGTGTA | |