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Figure 2 | AMB Express

Figure 2

From: Highly conserved salt bridge stabilizes a proteinase K subfamily enzyme, Aqualysin I, from Thermus aquaticus YT-1

Figure 2

Heat stability of the wild-type and mutant enzymes. Heat stability was determined as described in the Materials and methods section. The remaining activities after heat treatment at 70°C (a, c and e) or 80°C (b, d and f) for the indicated times relative to the activity before heat treatment are plotted for each mutant. (a, b) mutants lacking an intrinsic salt bridge, (c, d) mutants lacking a common salt bridge, (e, f) mutants in which a salt bridge may have been introduced.

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