Table 8 Central composite design for the defined medium
Run | Coded factors | AMVL (mg/L) | ||||
|---|---|---|---|---|---|---|
A | B | C | D | Actual | Predicted | |
1 | −1 | −1 | −1 | −1 | 157.6 ± 7.6 | 143.0 |
2 | 1 | −1 | −1 | −1 | 141.9 ± 6.3 | 130.3 |
3 | −1 | 1 | −1 | −1 | 71.0 ± 3.7 | 68.2 |
4 | 1 | 1 | −1 | −1 | 43.0 ± 2.4 | 55.6 |
5 | −1 | −1 | 1 | −1 | 117.3 ± 5.9 | 113.9 |
6 | 1 | −1 | 1 | −1 | 49.1 ± 2.2 | 46.1 |
7 | −1 | 1 | 1 | −1 | 81.2 ± 3.5 | 80.9 |
8 | 1 | 1 | 1 | −1 | 21.8 ± 2.6 | 13.1 |
9 | −1 | −1 | −1 | 1 | 68.2 ± 3.2 | 74.9 |
10 | 1 | −1 | −1 | 1 | 177.4 ± 9.1 | 173.9 |
11 | −1 | 1 | −1 | 1 | 38.2 ± 3.3 | 37.5 |
12 | 1 | 1 | −1 | 1 | 135.1 ± 7.4 | 136.5 |
13 | −1 | −1 | 1 | 1 | 85.3 ± 4.0 | 84.4 |
14 | 1 | −1 | 1 | 1 | 143.3 ± 7.5 | 128.4 |
15 | −1 | 1 | 1 | 1 | 94.8 ± 4.3 | 88.8 |
16 | 1 | 1 | 1 | 1 | 106.4 ± 5.7 | 132.8 |
17 | −2 | 0 | 0 | 0 | 80.5 ± 3.6 | 86.1 |
18 | 2 | 0 | 0 | 0 | 122.1 ± 5.2 | 117.4 |
19 | 0 | −2 | 0 | 0 | 86.0 ± 4.4 | 103.2 |
20 | 0 | 2 | 0 | 0 | 49.1 ± 3.5 | 32.8 |
21 | 0 | 0 | −2 | 0 | 171.9 ± 8.6 | 167.8 |
22 | 0 | 0 | 2 | 0 | 139.9 ± 7.4 | 134.9 |
23 | 0 | 0 | 0 | −2 | 19.8 ± 2.6 | 30.3 |
24 | 0 | 0 | 0 | 2 | 91.4 ± 5.8 | 81.8 |
25 | 0 | 0 | 0 | 0 | 141.9 ± 7.4 | 151.3 |
26 | 0 | 0 | 0 | 0 | 135.8 ± 6.1 | 151.3 |
27 | 0 | 0 | 0 | 0 | 154.2 ± 7.3 | 151.3 |
28 | 0 | 0 | 0 | 0 | 168.5 ± 8.7 | 151.3 |
29 | 0 | 0 | 0 | 0 | 149.4 ± 7.0 | 151.3 |
30 | 0 | 0 | 0 | 0 | 158.3 ± 8.2 | 151.3 |