Skip to main content

Table 8 Central composite design for the defined medium

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Run Coded factors AMVL (mg/L)
A B C D Actual Predicted
1 −1 −1 −1 −1 157.6 ± 7.6 143.0
2 1 −1 −1 −1 141.9 ± 6.3 130.3
3 −1 1 −1 −1 71.0 ± 3.7 68.2
4 1 1 −1 −1 43.0 ± 2.4 55.6
5 −1 −1 1 −1 117.3 ± 5.9 113.9
6 1 −1 1 −1 49.1 ± 2.2 46.1
7 −1 1 1 −1 81.2 ± 3.5 80.9
8 1 1 1 −1 21.8 ± 2.6 13.1
9 −1 −1 −1 1 68.2 ± 3.2 74.9
10 1 −1 −1 1 177.4 ± 9.1 173.9
11 −1 1 −1 1 38.2 ± 3.3 37.5
12 1 1 −1 1 135.1 ± 7.4 136.5
13 −1 −1 1 1 85.3 ± 4.0 84.4
14 1 −1 1 1 143.3 ± 7.5 128.4
15 −1 1 1 1 94.8 ± 4.3 88.8
16 1 1 1 1 106.4 ± 5.7 132.8
17 −2 0 0 0 80.5 ± 3.6 86.1
18 2 0 0 0 122.1 ± 5.2 117.4
19 0 −2 0 0 86.0 ± 4.4 103.2
20 0 2 0 0 49.1 ± 3.5 32.8
21 0 0 −2 0 171.9 ± 8.6 167.8
22 0 0 2 0 139.9 ± 7.4 134.9
23 0 0 0 −2 19.8 ± 2.6 30.3
24 0 0 0 2 91.4 ± 5.8 81.8
25 0 0 0 0 141.9 ± 7.4 151.3
26 0 0 0 0 135.8 ± 6.1 151.3
27 0 0 0 0 154.2 ± 7.3 151.3
28 0 0 0 0 168.5 ± 8.7 151.3
29 0 0 0 0 149.4 ± 7.0 151.3
30 0 0 0 0 158.3 ± 8.2 151.3
  1. Note: A, NaNO3; B, K2HPO4; C, KH2PO4; D, Trace elements.