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Table 5 Coded and uncoded values of independent variables used in the central composite design for the complex and defined media

From: Assessing medium constituents for optimal heterologous production of anhydromevalonolactone in recombinant Aspergillus oryzae

Independent variables Coded value
-α(−2) −1 0 +1 +α(+2)
Complex medium      
A, Sucrose (g/L) 10 40 70 100 130
B, NaNO3 (g/L) 1 3 5 7 9
C, Yeast extract (g/L) 1 3 5 7 9
D, K2HPO4 (g/L) 1 3 5 7 9
Defined medium      
A, NaNO3 (g/L) 3 5 7 9 11
B, K2HPO4 (g/L) 1 2 3 4 5
C, KH2PO4 (g/L) 0 0.5 1 1.5 2
D, Trace elements (mL/L) 0 0.15 0.30 0.45 0.60