Table 5 Coded and uncoded values of independent variables used in the central composite design for the complex and defined media
Independent variables | Coded value | ||||
|---|---|---|---|---|---|
-α(−2) | −1 | 0 | +1 | +α(+2) | |
Complex medium | Â | Â | Â | Â | Â |
A, Sucrose (g/L) | 10 | 40 | 70 | 100 | 130 |
B, NaNO3 (g/L) | 1 | 3 | 5 | 7 | 9 |
C, Yeast extract (g/L) | 1 | 3 | 5 | 7 | 9 |
D, K2HPO4 (g/L) | 1 | 3 | 5 | 7 | 9 |
Defined medium | Â | Â | Â | Â | Â |
A, NaNO3 (g/L) | 3 | 5 | 7 | 9 | 11 |
B, K2HPO4 (g/L) | 1 | 2 | 3 | 4 | 5 |
C, KH2PO4 (g/L) | 0 | 0.5 | 1 | 1.5 | 2 |
D, Trace elements (mL/L) | 0 | 0.15 | 0.30 | 0.45 | 0.60 |