Table 1 Primer sets and sequences used for the amplification of the partial 16S rDNA sequences by qPCR
From: Absolute Quantification of Individual Biomass Concentrations in a Methanogenic Coculture
Primer name | Target | Primer sequence (5’-3’) | Reference |
|---|---|---|---|
DSVsp G11 201f | Desulfovibrio sp. strain G11 | GACCTCTGCTTGCATGTTACC | This study |
DSVsp G11 471r | CTGATTAGCACAGTGCGGTTT | This study | |
Arch25f | Archaea | CTG GTT GAT CCT GCC AG | Mariakakis et al. ([2011]) |
MH236r | Methanospirillum hungatei JF1 | CAG ACT CAT CCT GAA GCG AC | Worm et al. ([2011]) |