Figure 2

UPLC-PDA analyses of epothilone A reaction mixture with different sugar donors revealed the new peak(s) generating their respective glycosides as novel compounds. The chromatogram (A) represents the reaction mixture with UDP-D-glucose; (B) TDP-D-2-deoxyglucose; (C) UDP-D-galactose, (D) GDP-L-fucose, and (E) and TDP-L-rhamnose with epothilone A. Reaction mixture with UDP-D-galactose generated two novel peaks and has been identified as mono-galactoside and di-galactoside of epothilone A. The triangle shape symbol represents epothilone A aglycon standard whereas the filled diamond shape symbols represent the peaks of rare sugars conjugated epothilone A glycosides.