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Table 1 Bacterial strains and plasmids

From: Disruption of pknG enhances production of gamma-aminobutyric acid by Corynebacterium glutamicum expressing glutamate decarboxylase

Bacterial strains or plasmids Relevant characteristics Reference or source
E. coli   
SCS110 rpsL (Strr) thr leu endA thi-l lacY galK galT ara tonA tsx dam dcm Stratagene
  supE44Δ (lac-proAB) [F’traD36 proAB laclqZΔM15]  
C. glutamicum   
ATCC 13032 Wild-type C. glutamicum, biotin-auxotrophic, L-glutamate producing strain ATCC
W Wild-type C. glutamicum derivative harboring pCH Takahashi et al. [2012]
GAD Wild-type C. glutamicum derivative harboring pCH-gadB Takahashi et al. [2012]
ΔpknG Wild-type C. glutamicum derivative with deletion in pknG This study
GADΔpknG Wild-type C. glutamicum derivative with deletion in pknG, harboring pCH-gadB This study
Plasmids   
pCH E. coli-C. glutamicum shuttle vector with HCE promoter, Kmr Tateno et al. [2007]
pCH-gadB pCH containing gadB from E.coli W3110, Kmr Takahashi et al. [2012]
pTM44 pHSG298 with B. subtilis sacB and C. glutamicum hom, Kmr Mimitsuka et al. [2007]
pTM44-ΔpknG pTM44 with 1,601-bp Sph I-Bam HI fragment with deletions of pknG, Kmr This study
  1. r antibiotic resistance.