Table 1 Bacterial strains and plasmids
Bacterial strains or plasmids | Relevant characteristics | Reference or source |
|---|---|---|
E. coli | ||
SCS110 | rpsL (Strr) thr leu endA thi-l lacY galK galT ara tonA tsx dam dcm | Stratagene |
supE44Δ (lac-proAB) [F’traD36 proAB laclqZΔM15] | ||
C. glutamicum | ||
ATCC 13032 | Wild-type C. glutamicum, biotin-auxotrophic, L-glutamate producing strain | ATCC |
W | Wild-type C. glutamicum derivative harboring pCH | Takahashi et al. [2012] |
GAD | Wild-type C. glutamicum derivative harboring pCH-gadB | Takahashi et al. [2012] |
ΔpknG | Wild-type C. glutamicum derivative with deletion in pknG | This study |
GADΔpknG | Wild-type C. glutamicum derivative with deletion in pknG, harboring pCH-gadB | This study |
Plasmids | ||
pCH | E. coli-C. glutamicum shuttle vector with HCE promoter, Kmr | Tateno et al. [2007] |
pCH-gadB | pCH containing gadB from E.coli W3110, Kmr | Takahashi et al. [2012] |
pTM44 | pHSG298 with B. subtilis sacB and C. glutamicum hom, Kmr | Mimitsuka et al. [2007] |
pTM44-ΔpknG | pTM44 with 1,601-bp Sph I-Bam HI fragment with deletions of pknG, Kmr | This study |