Enhancing cellulase and hemicellulase production by genetic modification of the carbon catabolite repressor gene, creA, in Acremonium cellulolyticus

AMB Express

Table 1 Characteristics of the A. cellulolyticus strains and plasmids used in this study

Strain or Plasmid	Description	References
Strain
A. cellulolyticus Y-94	Wild type (FERM BP-5826)	Yamanobe et al. (1987)
YP-4	Uracil auxotroph mutant derived from Y-94	Inoue et al. (2013)
YDCre	YP-4 prototrophic transformant horboring pDCre2500, creA disruptant.	This study
YPyrF	YP-4 prototrophic transformant horboring a single copy pbs-pyrF in pyrF loci.	This study
YCreGFP	YP-4 prototrophic transformant harboring pCreGFP	This study
Plasmid
pbs-pyrF	Amp^r PyrF^r; pBluescript KS(+) derivative containing 2.7-kb fragment harboring pyrF from Y-94	Fujii et al. (2012)
pDCre2500	Amp^r PyrF^r; pbs-pyrF derivative containing 2.5 kb upstream and 2.5 kb downstream regions of creA	This study
pDCre1000	Amp^r PyrF^r; pbs-pyrF derivative containing 1.0 kb upstream and 1.0 kb downstream regions of creA	This study
pCreGFP	Amp^r PyrF^r; pbs-pyrF derivative containing GFP and creA fused gene	This study