Direct production of organic acids from starch by cell surface-engineered Corynebacterium glutamicum in anaerobic conditions

AMB Express

Table 3 Production of organic acids from starch by C. glutamicum cells in repeated cycles

Cycle	Start OD ₆₀₀	Consumed starch (g/l)	α-Amylase activity (U/g dry weight cell)		Concentration (g/l)				Yield ^a
Cycle	Start OD ₆₀₀	Consumed starch (g/l)	0 h	10 h	Lactate	Succinate	Acetate	Total organic acids	Yield ^a
1	159	39.5	15.4	9.4	27.6	3.9	1.3	32.8	0.83
2	122	27.5	9.5	7.2	19.5	2.8	0.8	23.2	0.84
3	103	23.0	7.0	5.4	15.6	2.4	0.9	18.9	0.82
4	99	21.2	7.8	5.4	14.4	2.7	1.1	18.3	0.86
5	91	17.3	6.8	5.0	11.8	2.2	0.8	14.8	0.86

^aYield is expressed as gram of total organic acids produced per gram of sugar consumed.
Each cycle was performed for 10 h at 30°C.