Figure 5

Optimal pH and temperature for the synthesis of 5-HTP, using the E. coli whole cell reaction. (a) The reaction conditions, except for pH and buffer, are described in the text. The following buffers were used: acetate buffer, pH 5.0–5.5; maleate buffer, pH 6.0; MOPS-NaOH buffer, pH 6.5; HEPES-NaOH buffer, pH 7.0–8.0; Tris–HCl buffer, pH 8.5; borate-NaOH buffer, pH 9.0–10. (b) The reaction conditions, except for temperature, are described in the text.