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AMB Express
Figure 2 | AMB Express

Figure 2

From: Evaluation of the food grade expression systems NICE and pSIP for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum

Figure 2

SDS-PAGE of cell free extracts of strains L. lactis NZ3900, Lb. plantarum TLG02 and Lb. plantarum WCFS1 cultivated without pH maintainance. Panel A: L. lactis NZ3900/pVK51dkr; Panel B: Lb. plantarum/pSIP603dkr; Panel C: Lb. plantarum/pSIP609dkr. Panel A: Lane 1 and Lane 9, molecular mass standard protein; Lane 2, culture uninduced; Lane 3-8, induced culture after 2, 4, 6, 8, 10 and 20 hours. Panel B, C: Lane 1 and Lane 9, molecular mass standard protein; Lane 2, culture uninduced; Lane 3-7, induced culture after 2, 4, 6, 8 and 10 hours; Lane 8, wild type Lb. plantarum WCFS1. The arrows indicate the band representing heterolougously expressed 2,5-DKG reductase.

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