AMB Express

Table 1 Summary of metagenomic data obtained from the mangrove microbiomes

From: A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of Brazilian mangroves

Parameters	Rio	Bahia
MG-RAST ID	44852183	44852193
No. of sequences	494,201	503,019
Avg. length (bp)	327 ± 110*	328 ± 111*
Total length (bp)	161,854,245	165,387,021
Predicted proteins ^†	488,630	450,087
Assigned reads	242,868	261,482
LCA ^¥
Bacteria (%)	206,733 (94,3)	273,078 (97,36)
Archaea (%)	10,041 (4,6)	4,623 (1,65)
Eukarya (%)	1,559 (0,7)	1,018 (0,36)
Viruses (%)	297 (0,1)	29 (0,01)
Unclassified (%)	649 (0,3)	1,740 (0,62)
MEGAN
No. of sequences	494,201	503,019
Assigned reads	293,374	340,668
Bacteria (%)	252,395 (93,6)	313,633 (97,3)
Archaea (%)	13,173 (4,88)	5,686 (1,76)
Eukarya (%)	3,295 (1,22)	2,805 (0,87)
Viruses (%)	554 (0,2)	57 (0,01)
Unclassified (%)	269 (0,1)	182 (0,06)

* After duplicate removal, splitting and trimming of sequence reads.
^†Predicted protein coding regions assigned an annotation using at least one of protein databases (M5NR) in MG-RAST server.
^¥Lowest common ancestor (LCA) using 1e-5 cutoff, 60% minimum identity, and a minimum alignment lenght cutoff of 15.

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