Targetsb
|
DNA sequencec
|
k
on
d (10-4 M-1 s-1)
|
k
off
c (10-3 s-1)
|
K
d
e (10-7 M)
|
---|
Control DNAf
|
5′bio-TCGTTTAACGAGCCCGTATTTTCCCCTCTACCTTTTAGAGGACACCTAAC-3′
|
0.4
|
0.7
|
18
|
phaP1 promoterg
|
5′bio-GGCGCATTTCTTATTTGGTGCGCCGCAACAATTCCTATTTTA GGGGCGCC-3′
|
6.0 ± 0.4
|
1.7 ± 0.4
|
3.2 ± 0.9
|
phaR promoterf
|
5′bio-TCACGCGTTTAGCCATAGCGGGCGCGGTA GACGAACAACAGCACGGCCGG-3′
|
0.5
|
0.9
|
18
|
am-P(3HB)g
| |
7.0 ± 3.8
|
-h
|
-h
|
-
a 10 mM HEPES buffer solution (pH 7.4) containing 150 mM NaCl and 0.002% Tween 20, 25°C. b 5′-biotinylated dsDNA immobilized on an avidin-covered QCM plate.
- Amorphous-P(3HB) thin film was prepared by casting chloroform solution (1.0 wt%) of the isotactic P[(R), (S)-3-hydroxybutyrate]. c The bold type indicates the DNA binding site of PhaR revealed by DNase I footprinting (4). d
k
on and k
off were obtained from Eq. 4. e
K
d was calculated as k
off/k
on. f The data are from 2 independent experiments. g Amorphous P(3HB); the data are from 3 independent experiments. h
K
d value could not be calculated due to a negative k
off value.