Figure 3
Transmission electron micrograph of Candida cells treated with cinnamaldehyde (50 μg/mL) and eugenol (200 μg/mL). (a) untreated; intact cell wall, cell membranes and other organelles. (b-f) treated with cinnamaldehyde; (b) disorganized protoplasm with receding of cell membrane (1), thickening of cell wall (2) (c) excessive vacuolization (v), loosening of cell membrane (3), disintegration of cell membrane (4) (d) invagination and disintegration of cell membrane (5), deposition of lipid globules (e) disintegrated cytoplasm and lysis of cell wall and membrane (7) (f) cell wall strength is lost and lysis of cellular material (8). (g-l) Treated with eugenol; (g) thickening of cell wall (9), loosening of cell membrane (10) (h) undulating cell wall (11) (i) deposition of lipid globules (12), disintegration of cell membrane (13) (j) excessive vacuolization (v), loosening of cell wall and cell membrane (14) (k) movement of cytoplasmic material to interconnected cell (15) (l) disintegrated cytoplasm, rupturing of cell membrane (16), swelling of cell wall (17).