Figure 2
Scanning electron micrograph of Candida cells treated with cinnamaldehyde (50 μg/mL) and eugenol (200 μg/mL). (a) untreated; oval shaped and smooth cell surfaces (b) untreated; arrow indicates polar bud scar. (c-e) treated with cinnamaldehyde; (c) shrinkage of cell membrane (i) scar on cell surface indicating lysis of cell membrane (ii) lysed and empty cells (iii) (d) deformed interconnected cells (i) with several bud scars without preferential position at the polar ends (arrows) (ii) deposits of lytic material is seen in the form of vesicles (V) (e) interconnected cells with convoluted surfaces, loosened cell membrane and receding of cytoplasmic material (i) lysed bud (ii). (f-i) treated with eugenol; (f) deformed cells with convoluted and irregular surfaces (i) (g) arrows indicate interconnected cells, lysed cell (i) (h) arrow indicates autolysis of cell (i) arrow indicates receding of cytoplasmic content from cell membrane and disintegration of cellular content.