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Table 1 Strains and plasmids used in this study

From: Mercurial-resistance determinants in Pseudomonas strain K-62 plasmid pMR68

Stains and plasmids Description or relevant feature(s) Reference or source
Strains
E. coli XL1-Blue recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 lac/ [F’::Tn 10 proAB+ lacI q lacZM15 traD36] (Bullock et al. 1987)
P. strain K-62 (wild) 82, 68,56, 31, 26, 8.5 kb plasmids IC50 of mercuric chloride; 100 ppm, mercury vapor activity; + Kiyono et al. (1995b)
P. strain K-62 (mutant 26) 82, 68, 56, 31, 8.5 kb plasmids IC50 of mercuric chloride; 50 ppm, mercury vapor activity; + Kiyono et al. (1995b)
P. strain K-62 (mutant 68) 82, 56, 31, 26, 8.5 kb plasmids IC50 of mercuric chloride; 17 ppm, mercury vapor activity; + Kiyono et al. (1995b)
P. strain K-62 (mutant TY) 82, 56, 31, 8.5 kb plasmids IC50 of mercuric chloride; 2 ppm, mercury vapor activity; - Kiyono et al. (1995b)
Plasmids
pMR26 26 kb plasmids from P. strain K-62 (Kiyono et al. 1997)
pMR68 68 kb plasmids from P. strain K-62 This study
pUC118 None; cloning vector (Vieira et al. 1987)
pMRA17 merR-o/p-merT-merP-merA-merG-merB1 of pMR26 in pBluescriptII (Kiyono et al. 1997)
pMRA114 merR-o/p-merT-merP-merA-merG-merB1 of pMR26 in pUC118 This study
pMKY12 merR-o/p-orf4-orf5-merT1-merP1-merA-merB1 of pMR68 in pUC118 This study