Table 1 Strains and plasmids used in this study
From: Mercurial-resistance determinants in Pseudomonas strain K-62 plasmid pMR68
Stains and plasmids | Description or relevant feature(s) | Reference or source | |
|---|---|---|---|
Strains | |||
E. coli XL1-Blue | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 lac/ [F’::Tn 10 proAB+ lacI q lacZM15 traD36] | (Bullock et al. 1987) | |
P. strain K-62 (wild) | 82, 68,56, 31, 26, 8.5 kb plasmids | IC50 of mercuric chloride; 100 ppm, mercury vapor activity; + | Kiyono et al. (1995b) |
P. strain K-62 (mutant 26) | 82, 68, 56, 31, 8.5 kb plasmids | IC50 of mercuric chloride; 50 ppm, mercury vapor activity; + | Kiyono et al. (1995b) |
P. strain K-62 (mutant 68) | 82, 56, 31, 26, 8.5 kb plasmids | IC50 of mercuric chloride; 17 ppm, mercury vapor activity; + | Kiyono et al. (1995b) |
P. strain K-62 (mutant TY) | 82, 56, 31, 8.5 kb plasmids | IC50 of mercuric chloride; 2 ppm, mercury vapor activity; - | Kiyono et al. (1995b) |
Plasmids | |||
pMR26 | 26 kb plasmids from P. strain K-62 | (Kiyono et al. 1997) | |
pMR68 | 68 kb plasmids from P. strain K-62 | This study | |
pUC118 | None; cloning vector | (Vieira et al. 1987) | |
pMRA17 | merR-o/p-merT-merP-merA-merG-merB1 of pMR26 in pBluescriptII | (Kiyono et al. 1997) | |
pMRA114 | merR-o/p-merT-merP-merA-merG-merB1 of pMR26 in pUC118 | This study | |
pMKY12 | merR-o/p-orf4-orf5-merT1-merP1-merA-merB1 of pMR68 in pUC118 | This study | |