Southern blot analyses of the Aaku80 -disruption mutants. (a) Diagram of Aaku80 disruption cassette 2. The candy-striped bars indicate the second intron of Aaku80. Results of Southern blot analyses using genomic DNA digested with (b) Bgl II (BII) and (c) Pst I (P). Lanes 1, 2, and 3 are MR12, coku80, and coku80rec strains, respectively.