Figure 3
From: Increasing protein production by directed vector backbone evolution
Functional study of the lacI repressor under induced (left bars) and non-induced (right bars) conditions employing pET28a(+)-CelA2, pET28a(+)M1-CelA2, pET22b(+)-BSLA and pET22b(+)M1-BSLA expression systems. Constructs with an M1 label harbor an epMEGAWHOP optimized vector backbone. Enzyme activity levels were determined with the corresponding screening systems in 96-well microtiter plate formats for CelA2 and BLSA. The reported values are the average of three 96-well microtiter plate measurements in which each hydrolase was expressed 8 times per plate and deviations are calculated from the corresponding mean values.