Activity studies and SDS-PAGE visualization of produced CelA2, BSLA, subtilisin Carlsberg wild type. a) Activity measurements are performed with not optimized (left column) and mutagenized vector backbones (right column) in 96-well microtiter plate. Constructs with an M1 label habor an epMEGAWHOP optimized vector backbone; b) Visualization of production levels of CelA2 (proteins in supernatant of lysate), BSLA and subtilisin Carlsberg (proteins in culture supernatant after precipitation) by SDS-PAGE in combination with Coomassie brilliant blue staining. The reported relative activity values are the average of three 96-well microtiter plate measurements in which each construct was expressed 8 times per plate. Deviations are calculated from the corresponding mean values.