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Table 3 Effect of variable glutaraldehyde concentrations on different quantities determined during calculation of the recovered activity of CLEAs of TLL (hydrolysis of triolein)*

From: Cross-linked enzyme aggregates (CLEAs) of selected lipases: a procedure for the proper calculation of their recovered activity

Mass of glutaraldehyde (mg) Total mass of CLEAs recovered Total mass of PP lost in supernatant and washings Mass of CLEAs assayed (aliquot) Mass of PP in aliquot of CLEAs assayed Activity of CLEAs Specific activity of CLEAs Recovered activity of CLEAs
  (mg) (mg) (mg) (mg) (μmol/min) (μmol/min*mg) (%)
10 68.7 1.35 13.1 4.3 9.5 2.2 3.5
20 83.4 0.7 12.7 3.5 11.4 3.2 5.2
40 98.2 0.07 10.3 2.5 14.4 5.8 9.2
50 95.5 0.57 11.4 2.8 19.5 7.0 11.1
60 110 6.7 12.5 1.9 9.2 4.8 7.7
80 128 4.3 13.3 2 8 3.9 6.3
  1. * Initial mass of PP used for CLEAs´ synthesis: 23.8 mg. Specific activity of native TLL under identical conditions: 62.9 μmol/min-mgPP.