Table 3 Effect of variable glutaraldehyde concentrations on different quantities determined during calculation of the recovered activity of CLEAs of TLL (hydrolysis of triolein)*
Mass of glutaraldehyde (mg) | Total mass of CLEAs recovered | Total mass of PP lost in supernatant and washings | Mass of CLEAs assayed (aliquot) | Mass of PP in aliquot of CLEAs assayed | Activity of CLEAs | Specific activity of CLEAs | Recovered activity of CLEAs |
|---|---|---|---|---|---|---|---|
(mg) | (mg) | (mg) | (mg) | (μmol/min) | (μmol/min*mg) | (%) | |
10 | 68.7 | 1.35 | 13.1 | 4.3 | 9.5 | 2.2 | 3.5 |
20 | 83.4 | 0.7 | 12.7 | 3.5 | 11.4 | 3.2 | 5.2 |
40 | 98.2 | 0.07 | 10.3 | 2.5 | 14.4 | 5.8 | 9.2 |
50 | 95.5 | 0.57 | 11.4 | 2.8 | 19.5 | 7.0 | 11.1 |
60 | 110 | 6.7 | 12.5 | 1.9 | 9.2 | 4.8 | 7.7 |
80 | 128 | 4.3 | 13.3 | 2 | 8 | 3.9 | 6.3 |