Figure 4From: Importance of the ammonia assimilation by Penicillium purpurogenum in amino derivative Monascus pigment, PP-V, production Silica gel TLC profiles of culture filtrate pigments and the effect of GS inhibitor on pigment production. PP-V or PP-O production medium cultures containing 0, 1 and 2Â mM MSX were cultured for 72Â hr. Pigments were extracted with EtOAc and resolved on silica gel TLC using developing solvent n-BuOH:AcOH:H2O (12:3:5). Violet pigment at Rf 0.75 was identified as PP-V. Orange pigment at Rf 0.65 was identified as PP-O.Back to article page