Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012

AMB Express

Table 2 Effect of Temperature, Time, and Agitation on ethanol production from glycerol

Run	Time (h)	Temperature (°C)	Agitation (rpm)	Ethanol (g/l)
1	24	30	120	7.48
2	48	30	120	11.72
3	72	30	120	15.53
4	24	35	120	11.51
5	48	35	120	17.02
6	72	35	120	18.83
7	24	40	120	12.56
8	48	40	120	16.96
9	72	40	120	17.97
10	24	30	160	4.15
11	48	30	160	9.11
12	72	30	160	12.88
13	24	35	160	8.01
14	48	35	160	12.9
15	72	35	160	15.34
16	24	40	160	10.24
17	48	40	160	13.31
18	72	40	160	13.88
19	24	30	200	9.52
20	48	30	200	14.92
21	72	30	200	16.65
22	24	35	200	12.76
23	48	35	200	16.97
24	72	35	200	17.19
25	24	40	200	13.71
26	48	40	200	16.86
27	72	40	200	17.32