Figure 4
Expression levels with respect to the concentration of inducer and time course of induction. Cultures of E. coli harboring pCM13(SELP-1020-A) in LB were induced with 0.5 mM or 1 mM of IPTG when the OD600 reached 1. Samplings were taken at the indicated intervals. Cell density is indicated below each lane with target recombinant protein indicated by arrows.