Table 1 List of primers and TaqMan probes for each targeted oral species with expected amplicon sizes
Primers or probes a, b | Sequence (5 ′ ➞3 ′) | Product size (bp) | Source |
|---|---|---|---|
S. oralis | |||
gtfR F | ACCAGCAGATACGAAAGAAGCAT | 235 | This study |
gtfR R | AGGTTCGGGCAAGCGATCTTTCT | ||
TaqMan probe | AAGGCTGCTGTTGCTGAAGAAGT | ||
S. gordonii | |||
gtfG F | CGGATGATGCTAATCAAGTGACC | 177 | This study |
gtfG R | GTTAGCTGTTGGATTGGTTGCC | ||
TaqMan probe | AGAACAGTCCGCTGTTCAGAGCAA | ||
V. parvula | |||
16S rRNA F | GGATAGATGAAAGGTGGCCTCT | 72 | This study |
16S rRNA R | CCAACTAGCTAATCAGACGCAAT | ||
LNA probe* | FAM-GAAGGAGG | ||
F. nucleatum | |||
fadA F | TGCAGCAAGTTTAGTAGGTG | 146 | This study |
fadA R | CATTGTAAACTTGTTCATTTTGT | ||
TaqMan probe | AGCACTAGATGCTGAATACCAA | ||
P. intermedia | |||
16S rRNA F | CGGTCTGTTAAGCGTGTTGTG | 100 | Loozen et al. |
16S rRNA R | CACCATGAATTCCGCATACG | ||
TaqMan probe | TGGCGGACTTGAGTGCACGC |