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Table 1 List of primers and TaqMan probes for each targeted oral species with expected amplicon sizes
| Primers or probes a, b | Sequence (5 ′ ➞3 ′) | Product size (bp) | Source |
|---|---|---|---|
| S. oralis | |||
| gtfR F | ACCAGCAGATACGAAAGAAGCAT | 235 | This study |
| gtfR R | AGGTTCGGGCAAGCGATCTTTCT | ||
| TaqMan probe | AAGGCTGCTGTTGCTGAAGAAGT | ||
| S. gordonii | |||
| gtfG F | CGGATGATGCTAATCAAGTGACC | 177 | This study |
| gtfG R | GTTAGCTGTTGGATTGGTTGCC | ||
| TaqMan probe | AGAACAGTCCGCTGTTCAGAGCAA | ||
| V. parvula | |||
| 16S rRNA F | GGATAGATGAAAGGTGGCCTCT | 72 | This study |
| 16S rRNA R | CCAACTAGCTAATCAGACGCAAT | ||
| LNA probe* | FAM-GAAGGAGG | ||
| F. nucleatum | |||
| fadA F | TGCAGCAAGTTTAGTAGGTG | 146 | This study |
| fadA R | CATTGTAAACTTGTTCATTTTGT | ||
| TaqMan probe | AGCACTAGATGCTGAATACCAA | ||
| P. intermedia | |||
| 16S rRNA F | CGGTCTGTTAAGCGTGTTGTG | 100 | Loozen et al. |
| 16S rRNA R | CACCATGAATTCCGCATACG | ||
| TaqMan probe | TGGCGGACTTGAGTGCACGC |
