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Table 1 Purification of MnP isoenzymes from Bjerkandera sp. (A), Phanerochaete chrysosporium (B), Physisporinus rivulosus (C), Phlebia radiata (D) and Phlebia sp. Nf b19 (E)

From: Screening of white-rot fungi manganese peroxidases: a comparison between the specific activities of the enzyme from different native producers

  Activity [U] Protein [mg] Specific activity [U/mg] Activity protein absorbance ratio [UL-1 absorbance at 280nm-1] Yield [%] Purification fold Size of MnP [kDa]
A
Culture fluid 38.0 2.9 13.3   100 1.0  
Ultrafiltration 19.8 2.6 7.6   52.2 0.6  
Mono Q BSMnP1 15.3 0.5 32.3 18 40.2 2.4 45
B
Culture fluid 38.7 1.447 26.7   100 1.0  
Ultrafiltration 6.9 0.403 17.0   17.7 0.6  
Mono Q PCMnP1 1.1 0.005 200.5 13 2.8 7.5 45
Mono Q PCMnP2 0.4 0.013 33.0 8 1.1 1.2 45
C
Culture fluid 36.6 2.685 13.6   100 1.0  
Ultrafiltration 7.7 0.278 27.6   20.9 2.0  
Mono Q PRMnP1 2.3 0.026 88.6 122 6.2 6.5 50
D
Culture fluid 52.0 4.669 11.2   100 1.0  
Ultrafiltration 24.6 2.713 9.1   47.2 0.8  
Mono QPradMnP1 5.8 0.245 23.7 33 11.1 2.1 50
Mono Q PradMnP2 4.0 0.199 19.9 21 7.6 1.8 50
Mono Q PradMnP3 7.2 0.234 30.8 33 13.8 2.8 50
E
Culture fluid 7.1 0.671 10.6   100 1.0  
Ultrafiltration 3.4 0.511 6.6   46.9 0.6  
Mono Q NFMnP1 0.7 0.012 55.2 67 9.3 5.2 49