Figure 3

(A-E) SDS Page of DhaA purification via IMAC. (A) Prestained Protein Ladder, (B) potassium phosphate buffer wash, (C) IMAC column elution with 20 mM imidazole, (D) IMAC column elution with 40 mM imidazole, (E) IMAC column elution with 500 mM imidazole; (F) ESI-MS of purified DhaA, measured m/z: 1351.17 [M + 28 H]28+, 1401.07 [M + 27 H]27+, 1454.98 [M + 26 H]26+, calculated mass using ApE - a plasmid editor: 37668 g mol^-1, measured deconvoluted mass 37805.59 g mol^-1.