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Figure 1 | AMB Express

Figure 1

From: A sensitive method for rapid detection of alkyl halides and dehalogenase activity using a multistep enzyme assay

Figure 1

General scheme of an enzymatic multistep assay for the detection of haloalkanes. The alcohol formed via dehalogenation (A) undergoes an enzymatic oxidation and H2O2 is liberated (B). Afterwards a peroxide-HRP complex oxidizes the chromogen ABTS (C) resulting in a measureable increase of absorbance at 405 nm (D). The enzymatic formation of primary aldehydes can alternatively be proven by precipitation with 2,4-DNPH (E) and a subsequent APCI-MS measurement of the resulting hydrazones (F).

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