Table 4 Summary of the purification steps of the β-glucosidase produced by F. proliferatum growing in corn stover + wheat bran based medium
From: The production of β-glucosidases by Fusarium proliferatum NBRC109045 isolated from Vietnamese forest
Step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification factor | Recovery (%) |
|---|---|---|---|---|---|
Crude extract | 150 | 9.38 | 16.0 | 1.0 | 100 |
(NH4)2SO4 | 106 | 1.98 | 53.5 | 3.3 | 70.4 |
DEAE Sepharose CL-6B | 48.6 | 0.33 | 148 | 9.2 | 32.3 |
Superdex75 | 23.8 | 0.08 | 288 | 18 | 15.8 |