Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

AMB Express

Table 1 Strains and plasmids used in this study

Strain or plasmid	Relevant properties	References
E. coli XL1 blue	Cloning and plasmid maintenance
E. coli DH10B(pAN2)	Plasmid methylation
E. coli BW 25113	Ipa operons expression
C. beijerinckii NRRL B593	Wild type, Isopropanol natural producer	(George et al. 1983 , Chen et al. 1986 )
C. acetobutylicum ATCC824		(Jones and Woods 1986 )
pMTL500E	Empty vector (ermB^R/amp^R)	(Oultram et al. 1988 )
-pFC002	thlp_[adh]	this article
-pFC005	thlp_[adh; adc]	this article
-pFC006	thlp_[adh; ctfA; ctfB]	this article
-pFC007	thlp_[adh; adc; ctfA; ctfB]	this article
pTHL	thlp_[thl], kan^R	this article
pAN2	ttc ^R	(Mermelstein and Papoutsakis1993, Heap et al.2007)