E. coli XL1 blue
|
Cloning and plasmid maintenance
| |
E. coli DH10B(pAN2)
|
Plasmid methylation
| |
E. coli BW 25113
|
Ipa operons expression
| |
C. beijerinckii NRRL B593
|
Wild type, Isopropanol natural producer
|
(George
et al.
1983
, Chen
et al.
1986
)
|
C. acetobutylicum ATCC824
| |
(Jones and Woods
1986
)
|
pMTL500E
|
Empty vector (ermBR/ampR)
|
(Oultram et al.
1988
)
|
-pFC002
|
thlp_[adh]
|
this article
|
-pFC005
|
thlp_[adh; adc]
|
this article
|
-pFC006
|
thlp_[adh; ctfA; ctfB]
|
this article
|
-pFC007
|
thlp_[adh; adc; ctfA; ctfB]
|
this article
|
pTHL
|
thlp_[thl], kanR
|
this article
|
pAN2
|
ttc
R
|
(Mermelstein and Papoutsakis1993, Heap et al.2007)
|