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Table 1 Strains and plasmids used in this study

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Strain or plasmid Relevant properties References
E. coli XL1 blue Cloning and plasmid maintenance  
E. coli DH10B(pAN2) Plasmid methylation  
E. coli BW 25113 Ipa operons expression  
C. beijerinckii NRRL B593 Wild type, Isopropanol natural producer (George et al. 1983 , Chen et al. 1986 )
C. acetobutylicum ATCC824   (Jones and Woods 1986 )
pMTL500E Empty vector (ermBR/ampR) (Oultram et al. 1988 )
-pFC002 thlp_[adh] this article
-pFC005 thlp_[adh; adc] this article
-pFC006 thlp_[adh; ctfA; ctfB] this article
-pFC007 thlp_[adh; adc; ctfA; ctfB] this article
pTHL thlp_[thl], kanR this article
pAN2 ttc R (Mermelstein and Papoutsakis1993, Heap et al.2007)
  1. Clostridium/E. coli selective marker are indicated in parentheses.