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Table 1 Strains and plasmids used in this study
| Strain or plasmid | Relevant properties | References |
|---|---|---|
| E. coli XL1 blue | Cloning and plasmid maintenance | |
| E. coli DH10B(pAN2) | Plasmid methylation | |
| E. coli BW 25113 | Ipa operons expression | |
| C. beijerinckii NRRL B593 | Wild type, Isopropanol natural producer | (George et al. 1983 , Chen et al. 1986 ) |
| C. acetobutylicum ATCC824 | (Jones and Woods 1986 ) | |
| pMTL500E | Empty vector (ermBR/ampR) | (Oultram et al. 1988 ) |
| -pFC002 | thlp_[adh] | this article |
| -pFC005 | thlp_[adh; adc] | this article |
| -pFC006 | thlp_[adh; ctfA; ctfB] | this article |
| -pFC007 | thlp_[adh; adc; ctfA; ctfB] | this article |
| pTHL | thlp_[thl], kanR | this article |
| pAN2 | ttc R | (Mermelstein and Papoutsakis1993, Heap et al.2007) |
