Multiple amino acid sequence alignment of CgPAD with functional PADs reported to date. The sequences used are L. brevis ATCC 367 (RM84) (Lb; ABJ63379), P. pentosaceus ATCC 25745 (Pp; AJ276891), L. plantarum WCFS1 (Lp; PDB code 2GC9; gi93279967), L. plantarum LPCHL2 (Ll; U63827), B. pumilus PS213 (Bap; X84815), B. subtilis 168 (Bs; AF017117), Enterobacter sp. Px6-4 (Px6-4; ACJ26748), and K. oxytoca (Ko; BAF65031). The sequences were aligned using the ClustalW program. Residues identical to those in CgPAD are shaded. Possible catalytic residues are indicated as open circles above the CgPAD sequence. Partially conserved Met residues are indicated as filled circle. Sequences of homologous hypothetical proteins of unknown function in the reported genome sequences are not included in the multiple alignments.