Table 1 Effect of several solvents, cations, inhibitors, reducing agents and metal chelators on the activity of PepP of Lactococcus lactis ssp. lactis DSM 20481

Solvents [% (v/v)]

Acetone

10

79

DMSO

10

51

EtOH

52

Cations² [mM]

Co²⁺

0.1

97

Sn²⁺

0.1

81

1

86

1

4

10

63

10

3

Cu²⁺

0.1

73

Zn²⁺

0.001

100

1

3

0.01

59

10

3

0.1

4

Mg²⁺

0.1

97

1

89

10

88

Reagents [mM]

β-mercaptoethanol²

0.1

100

Pepstatin A³

0.001

113

1

97

0.01

111

10

106

0.1

111

DTT²

0.1

99

1,10- phenanthroline⁴

0.1

103

1

84

1

96

10

51

10

53

EDTA²

0.1

105

30

13

1

102

PMSF⁵

0.1

120

10

100

1

114

30

1

10

10

Imidazole²

0.1

112

1

107

10

109