Production, active staining and gas chromatography assay analysis of recombinant aminopeptidase P from Lactococcus lactis ssp. lactis DSM 20481

AMB Express

Table 1 Effect of several solvents, cations, inhibitors, reducing agents and metal chelators on the activity of PepP of Lactococcus lactis ssp. lactis DSM 20481

	Substance	Concentration	Activity¹[%]	Substance	Concentration	Activity¹[%]
Solvents [% (v/v)]	Acetone	10	79	DMSO	10	51
Solvents [% (v/v)]	EtOH	10	52
Cations² [mM]	Co²⁺	0.1	97	Sn²⁺	0.1	81
		1	86		1	4
		10	63		10	3
	Cu²⁺	0.1	73	Zn²⁺	0.001	100
		1	3		0.01	59
		10	3		0.1	4
	Mg²⁺	0.1	97
		1	89
		10	88
Reagents [mM]	β-mercaptoethanol²	0.1	100	Pepstatin A³	0.001	113
		1	97		0.01	111
		10	106		0.1	111
	DTT²	0.1	99	1,10- phenanthroline⁴	0.1	103
		1	84		1	96
		10	51		10	53
	EDTA²	0.1	105		30	13
		1	102	PMSF⁵	0.1	120
		10	100		1	114
		30	1		10	10
	Imidazole²	0.1	112
	Imidazole²	1	107
		10	109

¹For each enzyme activity test an individual 100 % value was determined in relation to the corresponding solvent.
²dissolved in H₂O_dd; ³dissolved in DMSO; ⁴dissolved in Acetone; ⁵dissolved in EtOH.
n = 3; all standard deviations < 5 %.