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Table 1 Effect of several solvents, cations, inhibitors, reducing agents and metal chelators on the activity of PepP of Lactococcus lactis ssp. lactis DSM 20481

From: Production, active staining and gas chromatography assay analysis of recombinant aminopeptidase P from Lactococcus lactis ssp. lactis DSM 20481

  Substance Concentration Activity1[%] Substance Concentration Activity1[%]
Solvents [% (v/v)] Acetone 10 79 DMSO 10 51
EtOH 52    
Cations2 [mM] Co2+ 0.1 97 Sn2+ 0.1 81
1 86 1 4
10 63 10 3
Cu2+ 0.1 73 Zn2+ 0.001 100
1 3 0.01 59
10 3 0.1 4
Mg2+ 0.1 97    
1 89   
10 88   
Reagents [mM] β-mercaptoethanol2 0.1 100 Pepstatin A3 0.001 113
1 97 0.01 111
10 106 0.1 111
DTT2 0.1 99 1,10- phenanthroline4 0.1 103
1 84 1 96
10 51 10 53
EDTA2 0.1 105 30 13
1 102 PMSF5 0.1 120
10 100 1 114
30 1 10 10
Imidazole2 0.1 112    
1 107    
   10 109    
  1. 1For each enzyme activity test an individual 100 % value was determined in relation to the corresponding solvent.
  2. 2dissolved in H2Odd; 3dissolved in DMSO; 4dissolved in Acetone; 5dissolved in EtOH.
  3. n = 3; all standard deviations < 5 %.