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Table 5 Fuel properties of biodiesel from Y. lipolytica grown on glucose and WCO

From: Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel

Property/Test Strain NCIM 3589 on glucose Strain NCIM 3589 on WCO Strain NCIM 3472 on WCO US biodiesel standards ASTM D6751 European biodiesel standards EN14214 Indian biodiesel standards IS15607
Visual test + + + NS + NS
Density (g cm -3)* 0.81 (0.87) 1.04(0.87) 1.19 (0.87) NS 0.8600-0.900 0.8600-0.900
Water content (vol %)* ND ND ND 0.05max 0.25max 0.03max
TAN (mg NaOH/g)* 0.2 2.8 2.3 0.8max 0.5max 0.5max
FFA (%)* 0.1 1.4 1.15 NS NS NS
Cu strip corrosion* Class 1a Class 1a Class 1a Class 3max Class 1max Class 1max
CN*** 56.6 50.8 59 47-65 51 min 51 min
Kinematic viscosity (40°C; mm2/s) *** 4.44 3.6 6.44 1.9-6.0 3.5-5.0 3.5-5.0
SN* 190.81 (194.48) 256.16 (249.4) 168.5 (177) NS NS NS
IV* 65.7 (70.64) 37.8 (47.9) 54.5 (61) NS 120max NS
HHV(M J kg-1)*** 40.39 36.77 41.25 NS NS NS
Concentration of γ-linolenic acid (C18:3) (%)* 0.1 0 0 NS 12max NS
FAME having ≥4 double bonds (%)* ND ND ND NS 1 max NS
  1. WCO – waste cooking oil. The experimental values are means of three independent determinations.
  2. ND: Not Detected; NS: Not Specified. * Experimentally determined values, followed by predicted ones, if any, in brackets.
  3. ** Calculated using experimental SN and IV while the values in brackets determined from predicted ones.
  4. *** Predicted values as mentioned in Materials and Methods.