Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel

AMB Express

Table 5 Fuel properties of biodiesel from Y. lipolytica grown on glucose and WCO

Property/Test	Strain NCIM 3589 on glucose	Strain NCIM 3589 on WCO	Strain NCIM 3472 on WCO	US biodiesel standards ASTM D6751	European biodiesel standards EN14214	Indian biodiesel standards IS15607
Visual test	+	+	+	NS	+	NS
Density (g cm ^-3)*	0.81 (0.87)	1.04(0.87)	1.19 (0.87)	NS	0.8600-0.900	0.8600-0.900
Water content (vol %)*	ND	ND	ND	0.05max	0.25max	0.03max
TAN (mg NaOH/g)*	0.2	2.8	2.3	0.8max	0.5max	0.5max
FFA (%)*	0.1	1.4	1.15	NS	NS	NS
Cu strip corrosion*	Class 1a	Class 1a	Class 1a	Class 3max	Class 1max	Class 1max
CN***	56.6	50.8	59	47-65	51 min	51 min
Kinematic viscosity (40°C; mm²/s) ***	4.44	3.6	6.44	1.9-6.0	3.5-5.0	3.5-5.0
SN*	190.81 (194.48)	256.16 (249.4)	168.5 (177)	NS	NS	NS
IV*	65.7 (70.64)	37.8 (47.9)	54.5 (61)	NS	120max	NS
HHV(M J kg^-1)***	40.39	36.77	41.25	NS	NS	NS
Concentration of γ-linolenic acid (C18:3) (%)*	0.1	0	0	NS	12max	NS
FAME having ≥4 double bonds (%)*	ND	ND	ND	NS	1 max	NS

WCO – waste cooking oil. The experimental values are means of three independent determinations.
ND: Not Detected; NS: Not Specified. * Experimentally determined values, followed by predicted ones, if any, in brackets.
** Calculated using experimental SN and IV while the values in brackets determined from predicted ones.
*** Predicted values as mentioned in Materials and Methods.