AMB Express

Table 1 HPLC retention times, pseudomolecular ions in HPLC-MS, and characteristics of the UV absorption spectra of [¹⁴C]PRA and its metabolites

From: Metabolite proving fungal cleavage of the aromatic core part of a fluoroquinolone antibiotic

Characteristic			Values for
	PRA	F-1	F-2	F-5	F-6	F-9	F-10 ^b	F-13
Retention time (min)
Method A	23.8	20.2	23.3	25.3	28.1	28.6	~2.4	15.2
Method B	32.0	24.7	31.4	35.2	41.0	38.7	~2.4	16.8
Pseudomolecular ion
[M+H]⁺ (m/z)^a	397	369	395	411	388	288	127	247
UV absorption (nm)
λ_max (major)	288	250	288	298	242	272	-	280
λ_max (minor)	364 248	278 362 374	376 392	260 374	298 330	328 350	-	320 362
Shoulder	374 340		260

a [M+H]⁺ was accompanied by [M+2+H]⁺, resulting from the ¹⁴C-label;
b Data obtained in an experiment with [pyrrolidinopiperidine-7-¹⁴C]PRA

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