Skip to main content

Advertisement

Table 1 HPLC retention times, pseudomolecular ions in HPLC-MS, and characteristics of the UV absorption spectra of [14C]PRA and its metabolites

From: Metabolite proving fungal cleavage of the aromatic core part of a fluoroquinolone antibiotic

Characteristic    Values for     
  PRA F-1 F-2 F-5 F-6 F-9 F-10 b F-13
Retention time (min)         
Method A 23.8 20.2 23.3 25.3 28.1 28.6 ~2.4 15.2
Method B 32.0 24.7 31.4 35.2 41.0 38.7 ~2.4 16.8
Pseudomolecular ion         
[M+H]+ (m/z)a 397 369 395 411 388 288 127 247
UV absorption (nm)         
   λmax (major) 288 250 288 298 242 272 - 280
   λmax (minor) 364 248 278 362 374 376 392 260 374 298 330 328 350 - 320 362
   Shoulder 374 340   260      
  1. a [M+H]+ was accompanied by [M+2+H]+, resulting from the 14C-label;
  2. b Data obtained in an experiment with [pyrrolidinopiperidine-7-14C]PRA