Effect of displayed mCherry on the Y. lipolytica transformants and culture media. ( A) Photograph of cells carrying mCherry-YlCWP1, the cells showed a pinkish-reddish colour. ( B) Photograph of the control cells carrying YlCWP1, cells did not show any colour change. ( C) Photograph of shake flask culture media containing Y. lipolytica displaying mCherry proteins, the culture medium showed a change in colour during the cultivation period which was a result of the displayed mCherry. ( D) Photograph of the shake flask culture media containing Y. lipolytica control cells, culture media did not show any change in colour during the cultivation period.