Multi-alignment of fungal chitosanases. The deduced amino acid sequences from 9 genes sequences were aligned by Mega 4. Amino acids that are identical between CSN and other sequence are shaded in black and the two conserve regions are underlined. The residue that was not identical but at least similar to the column-consensus be printed in another user-defined rendition usually on gray background. Vertical arrows indicate essential amino acid residues for catalytic activity of fungal chitosanases. GenBank accession details are for Penicillium sp. D-1 (this study), Talaromyces stipitatus (B8M2R4), Penicillium marneffei (B6QAJ8), Aspergillus clavatus (A1CN44), Neosartorya fischeri (A1DKV7), N. fumigata (Q4W904), A. fumigatus (B0YDW3), Fusarium solani subsp. Phaseoli (Q00867), F. solani f. robiniae (Q8NK77) and Colletotrichum graminicola (E3QWY0).