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Table 3 Optimization of ratios of fungal conidia to bacterial cells and co-cultivation periods on IM plates

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Number of
conidia
OD660 of
Agrobacterium
culture
Ratio of conidia:
Agrobacterium
Mean of transformants ± SD/104
conidia
 
    24 h 48 h 72 h
1 × 104 0.2 1:2.5 × 103 n.d.b 8 ± 7 (n = 4) n.d.
1 × 104 0.4 1:5 × 103 n.d. 7 ± 6 (n = 6) n.d.
1 × 104 0.6 1:7.5 × 103 n.d. 10 ± 7 (n = 4) n.d.
1 × 104 0.8 1:104 1 ± 1 (na = 2) 30 ± 28 (n = 12) 34 ± 27 (n = 2)
1 × 104 1.0 1:1.25 × 103 n.d. 62 ± 20 (n = 2) n.d.
  1. a n, number of independent experiments.
  2. bn.d., not done.