Skip to main content

Table 3 Optimization of ratios of fungal conidia to bacterial cells and co-cultivation periods on IM plates

From: Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Number of

conidia

OD660 of

Agrobacterium

culture

Ratio of conidia:

Agrobacterium

Mean of transformants ± SD/104

conidia

 
   

24 h

48 h

72 h

1 × 104

0.2

1:2.5 × 103

n.d.b

8 ± 7 (n = 4)

n.d.

1 × 104

0.4

1:5 × 103

n.d.

7 ± 6 (n = 6)

n.d.

1 × 104

0.6

1:7.5 × 103

n.d.

10 ± 7 (n = 4)

n.d.

1 × 104

0.8

1:104

1 ± 1 (na = 2)

30 ± 28 (n = 12)

34 ± 27 (n = 2)

1 × 104

1.0

1:1.25 × 103

n.d.

62 ± 20 (n = 2)

n.d.

  1. a n, number of independent experiments.
  2. bn.d., not done.