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AMB Express

Table 2 Effect of inhibitors, metal ions and solvents on AS-protease activity.

From: Identification and characterization of alkaline serine protease from goat skin surface metagenome

Additives

Relative activity (%)

None

100

PMSF (5 mM)

22

EDTA (5 mM)

100

DTT (5 mM)

38

β-ME (5 mM)

38

DMSO (1%)

34

SDS (0.5%)

26

Iso-propanol (1%)

125

MnCl2 (5 mM)

200

CaCl2 (5 mM)

138

CoCl2 (5 mM)

225

NiSO4 (5 mM)

109

FeSO4 (5 mM)

27

HgCl2 (5 mM)

113

ZnCl2 (5 mM)

94

  1. The purified AS-protease was preincubated with inhibitors, metal ions or additives/solvents for 15 min at 37°C. The activity of protease measured without any additive was set as 100%.
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