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Table 2 Effect of inhibitors, metal ions and solvents on AS-protease activity.

From: Identification and characterization of alkaline serine protease from goat skin surface metagenome

Additives Relative activity (%)
None 100
PMSF (5 mM) 22
EDTA (5 mM) 100
DTT (5 mM) 38
β-ME (5 mM) 38
DMSO (1%) 34
SDS (0.5%) 26
Iso-propanol (1%) 125
MnCl2 (5 mM) 200
CaCl2 (5 mM) 138
CoCl2 (5 mM) 225
NiSO4 (5 mM) 109
FeSO4 (5 mM) 27
HgCl2 (5 mM) 113
ZnCl2 (5 mM) 94
  1. The purified AS-protease was preincubated with inhibitors, metal ions or additives/solvents for 15 min at 37°C. The activity of protease measured without any additive was set as 100%.