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Table 1 List of bacterial strains and plasmids used in this study

From: Identification and characterization of alkaline serine protease from goat skin surface metagenome

Strains/plasmids Genotype/Description Reference/Source
E. coli DH5α F- end A1 gln V44 thi- 1 rec A1 rel A1 gyr A96 deo R nup G Φ80dlacM15 Δ(lac ZYA-arg F) U169, hsd R17(r K - m K + ), λ- Invitrogen (CA, USA)
E. coli DH10B F- end A1 rec A1 gal E15 gal K16 nupG rps L Δlac X74 Φ80lac ZΔM15 ara D139 Δ(ara, leu) 7697 mcr A Δ(mrr-hsd RMS-mcr BC) λ- Invitrogen (CA, USA)
E. coli BL21 (DE3) F- omp T gal dcm lon hsd SB (r B - m B - ) λ( DE3 [lacI lac UV5-T7 gene 1 ind 1 sam 7 nin 5]) Novagen (CA, USA)
pUC19 Apr ; Cloning vector Stratagene (CA, USA)
pTZ57R/T Apr; PCR cloning vector MBI Fermentas (Opelstrasse, Germany)
pET30b Knr; Expression vector; T7 promoter Novagen (CA, USA)
pSP1 pUC19 harbouring the AS-protease ORF; Apr This study
pTSP1 AS- Protease ORF cloned in pTZ57R/T; Apr This study
pETP1 AS- protease ORF cloned in pET30b; Knr This study