A simple method to evaluate the number of bradyrhizobia on soybean seeds and its implication on inoculant quality control

AMB Express

Table 1 Minimal inhibitory concentration (MIC) of 16 Rhizobiales strains

Scientific nomenclature	Strain	Origin	MIC (μg.ml ^-1 ) ^(*)
Rhizobium leguminosarum biovar viceae	D36	WDCM31⁽¹⁾	>16
Rhizobium leguminosarum biovar viceae	175G10b	Merck Crop Bioscience⁽²⁾	2
Rhizobium leguminosarum biovar trifolii	162BB1	WDCM31⁽¹⁾	4
Rhizobium leguminosarum biovar trifolii	162P17	WDCM31⁽¹⁾	2
Bradyrhizobium japonicum	E109	WDCM31⁽¹⁾	>16
Bradyrhizobium japonicum	61A227	Merck Crop Bioscience⁽²⁾	>16
Bradyrhizobium japonicum	61A228	Merck Crop Bioscience⁽²⁾	>16
Bradyrhizobium japonicum	61A273	Merck Crop Bioscience⁽²⁾	>16
Bradyrhizobium spp.	8A57	Merck Crop Bioscience⁽²⁾	>16
Mesorhizobium huakii	LL32	WDCM31⁽¹⁾	4
Rhizobium loti	95C14	Merck Crop Bioscience⁽²⁾	16
Rhizobium loti	95C11	Merck Crop Bioscience⁽²⁾	4
Sinorhizobium meliloti	B401	WDCM31⁽¹⁾	>16
Sinorhizobium meliloti	102F77b	Merck Crop Bioscience⁽²⁾	16
Sinorhizobium meliloti	102F51a	Merck Crop Bioscience⁽²⁾	8
Sinorhizobium meliloti	102F34a	Merck Crop Bioscience⁽²⁾	16

Bacterial nomenclature, origin and vancomycin minimal inhibitory concentration (MIC) corresponding to 16 Rhizobiales strains selected for this work. The MIC methodology was performed according to Clinical and Laboratory Standards Institute (CLSI) recommendations.
^(*) Minimal Inhibitory Concentration (MIC) for vamcomycin
⁽¹⁾ Corresponding to IMYZA-Instituto de Microbiología y Zoología Agrícola. Castelar. Argentina
⁽²⁾ Currently deposited in CCM-A WDCM29