Expression of KmINU1 in various sugars under a static condition. Cells grown in YPD medium for 18 h were inoculated into YP supplemented with 2% glucose (YPD), sucrose (YPSuc), raffinose (YPRaf) or inulin (YPInu), or a mixture of these sugars with 2% glucose, and cultivated further for 4 h at 30°C or 45°C. Total RNA was then isolated and subjected to RT-PCR with primers specific to corresponding genes that amplify an approximately 500-bp DNA fragment. (a) After reverse transcriptase reaction, PCR products of 20, 25, 30 and 35 cycles were subjected to 0.9% agarose gel electrophoresis and stained with ethidium bromide. (b) Relative band intensities were determined using scanned image and UN-SCAN-IT software (Silk Scientific, Orem, UT, U.S.A.).